Identification of Novel Begomoviruses Associated with Leaf Curl Disease of Papaya (Carica papaya L.) in India

نویسندگان

چکیده

Papaya (Carica papaya L.) is one of the most important fruit crops grown in tropical and subtropical regions world. leaf curl disease greatest concerns next to ring spot for India A survey was conducted during year 2019 2021 assessing incidence five major papaya-growing districts Karnataka State, India. The ranged from 10 21 percent, with plants expressing typical begomovirus symptoms. Thirty-two virus-infected samples (PLC-1 PLC-32), collected different farmer’s fields, gave positive amplification detection. Based on partial genome analysis, 13 representative isolates were selected complete by rolling circle DNA (RCA). RCA products cloned, sequenced analyzed. analysis strain classification criteria begomoviruses, (PLC-2, 3, 9, 11 18) considered variants Chilli virus (ChiLCV). Isolate PLC-22 a ChiLCV, 93.5% nt identity sharing. Similarly, isolate PLC-28 Croton yellow vine mosaic (CYVMV), PLC-25 PLC-31 as strains (PaLCuV). Among remaining four isolates, three (PLC-1, PLC-4 PLC-7) share more than 91% among them less all other reported isolates. Hence, they are be novel begomovirus, name Bagalkote [India:Karnataka:Bagalkote:Papaya:2021] proposed. One (PLC-32) also found distinct including current study which we propose Haveri [India:Karnataka:Haveri:Papaya:2021]. putative recombination showed that part viral likely descended begomoviruses previously. This first report diversity distribution infecting Karnataka, investigation results revealed papaya-infecting (PaLCuBKV, PaLCuV, CYVMV PaLCuHV) sampled regions.

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ژورنال

عنوان ژورنال: Agronomy

سال: 2022

ISSN: ['2156-3276', '0065-4663']

DOI: https://doi.org/10.3390/agronomy13010003